Rose Hybridizers Association Forum

Thanks very much Dr. Kuska. So the answer is that they swapped out the promotor genes that control the genes for a pair of dna repair proteins. The native promotor acted during both mieosis and mitosis whilst the replacement promotor acts only during mitosis. Such molecular manipulations are becomin...

This paper by a team of researchers from several different universities has found a way to break the species barrier for making hybrid crosses. They somehow inhibited the expression of a pair of dna repair proteins during mieosis "whilst maintaining their mitotic expression". The 'somehow' is not st...

If someone wants to do the experiment I can pony up pollen that's been frozen for five years, or maybe more. I suspect properly dried, frozen pollen would last decades. In a lot of ways germination of pollen resembles seed germination. I once successfully germinated rose seeds that had been in a USD...

They have upped their game. https://www.nature.com/articles/s41598-020-70469-1 The protocol here uses the old school shotgun technique and they did apparently transform true leaves. Then they neutralized the virus with heat after the transformation, claiming it results in pathogenically benign breed...

This 2014 paper is ancient news in the world of genetics but news to me none-the-less. These folks took a couple of genes from arabidopsis, the white rat of plant research, and injected them into apple seedling cotyledons: " When apple cotyledons were inoculated with ALSV-AtFT/MdTFL1 immediately aft...

As in full blown rescue using tissue culture? Do you have a protocol you can share with us, photos maybe?

>> Since in our climate seeds do not ripen for independent germination, we had to master the embryo rescue.

Where are you?

Otherwise known as Dancing Roots. https://youtu.be/uXwYKymTiQg These folks germinated some seeds in a gel medium then recorded the root growth on a 15 minute time lapse. The one on the right is a mutant that doesn't do the dance. See: https://phys.org/news/2021-02-time-lapse-reveals-hidden-roots.htm...

>> I'm sure they were delighted that the transgenes aren't heritable. The chimerism may not have simply been fortuitous. The original engineering for the blue rose occurred more than two decades ago iirc. Assuming the transformation was heritable, how difficult would it be to subsequently create a c...

Hmmmm, I wonder if "PKO" has been trademarked in the EU?

Someone could challenge the patent head on: "If the patent has already been issued by the USPTO, then a third party can challenge the patent at the USPTO by filing a request for reexamination of the patent. An ex parte reexamination can be filed by any person." http://www.trademarklawyerwestpalmbeac...

For roses there is no bottom line answer to the question of whether ploidy is the cause of a failed cross because there are other possible reasons for failure. Chief among these is the problem of self-sterility. Moreover, it is pretty much impossible to ensure that any given successful cross is free...

The fact that they germinated in tandem and were juxtaposed lends support to them being 'twins', two embryos in the same seed. Time will tell.

What are they?

Drop the temp back to ice cold but not freezing for a few days then raise it to to 45 to 55 degrees and hold them there.

As Joe suggests you should check a few to make sure that you have viable embryos.

Where in Massachusetts are you?

Very interesting. Very, very rare,do whatever it takes to keep them alive.

>> Joe Winchell and Ralph Moore applied for unpaid internships with Burbank? He rejected both, stating he felt neither "would ever amount to anything". Hmm. I'm thinking Ralph might have spun that yarn a little thin but I guess it's possible. Ralph was born right at the beginning of 1907 while Joe w...

One question, and I don't know the answer:

What thornless rose grows in the garden of Luther Burbank's former home in Santa Rosa, California and did he breed it? (ok, that's two questions.)

https://en.wikipedia.org/wiki/Luther_Burbank

Here is an article on the branding situation with apples which parallels somewhat the situation with the horticultural industry. You'll note that the apple branding competition started with the abandonment of the apple growers cooperative marketing effort at the turn of the century, a situation that...

Or should I say Stress Memory Test? Researchers Surprised After They Graft Tomato Plants With Epigenetically-Modified Rootstock https://scitechdaily.com/researchers-surprised-after-they-graft-tomato-plants-with-epigenetically-modified-rootstock/ At first read this article seemed like a flashback to ...

If they are 100 days old or close to it then they are mature enough to harvest. Otherwise yes. Leave the lights on 24/7.